Overview

The csb.bio.sequence module defines the base interfaces of our sequence and sequence alignment objects: AbstractSequence and AbstractAlignment. This module provides also a number of useful enumerations, like SequenceTypes and SequenceAlphabets.

Sequences

AbstractSequence has a number of implementations. These are of course interchangeable, but have different intents and may differ significantly in performance. The standard Sequence implementation is what you are after if all you need is high performance and efficient storage (e.g. when you are parsing big files). Sequence objects store their underlying sequences as strings. RichSequences on the other hand will store their residues as ResidueInfo objects, which have the same basic interface as the csb.bio.structure.Residue objects. This of course comes at the expense of degraded performance. A ChainSequence is a special case of a rich sequence, whose residue objects are actually real csb.bio.structure.Residues.

Basic usage:

>>> seq = RichSequence('id', 'desc', 'sequence', SequenceTypes.Protein)
>>> seq.residues[1]
<ResidueInfo [1]: SER>
>>> seq.dump(sys.stdout)
>desc
SEQUENCE 

See AbstractSequence in the API docs for details.

Alignments

AbstractAlignment defines a table-like interface to access the data in an alignment:

>>> ali = SequenceAlignment.parse(">a\nABC\n>b\nA-C")
>>> ali[0, 0]
<SequenceAlignment>   # a new alignment, constructed from row #1, column #1
>>> ali[0, 1:3]
<SequenceAlignment>   # a new alignment, constructed from row #1, columns #2..#3

which is just a shorthand for using the standard 1-based interface:

>>> ali.rows[1]
<AlignedSequenceAdapter: a, 3>                        # row #1 (first sequence)
>>> ali.columns[1]
(<ColumnInfo a [1]: ALA>, <ColumnInfo b [1]: ALA>)    # residues at column #1

See AbstractAlignment in our API docs for all details and more examples.

There are a number of AbstractAlignment implementations defined here. SequenceAlignment is the default one, nothing surprising. A3MAlignment is a more special one: the first sequence in the alignment is a master sequence. This alignment is usually used in the context of HHpred. More important is the StructureAlignment, which is an alignment of csb.bio.structure.Chain objects. The residues in every aligned sequence are really the csb.bio.structure.Residue objects taken from those chains.

Sequence and Alignment I/O

CSB provides parsers and writers for sequences and alignments in FASTA format, defined in csb.bio.io.fasta. The most basic usage is:

>>> parser = SequenceParser()
>>> parser.parse_file('sequences.fa')
<SequenceCollection>   # collection of L{AbstractSequence}s

This will load all sequences in memory. If you are parsing a huge file, then you could efficiently read the file sequence by sequence:

>>> for seq in parser.read('sequences.fa'):
        ...            # seq is an L{AbstractSequence}


BaseSequenceParser is the central class in this module, which defines a common infrastructure for all sequence readers. SequenceParser is a standard implementation, and PDBSequenceParser is specialized to read FASTA sequences with PDB headers.

For parsing alignments, have a look at SequenceAlignmentReader and StructureAlignmentFactory.

Finally, this module provides a number of OutputBuilders, which know how to write AbstractSequence and AbstractAlignment objects to FASTA files:

>>> with open('file.fa', 'w') as out:
        builder = OutputBuilder.create(AlignmentFormats.FASTA, out)
        builder.add_alignment(alignment)
        builder.add_sequence(sequence)
        ...

or you could instantiate any of the OutputBuilders directly.

Last edited Oct 2, 2013 at 8:29 AM by kalev, version 2

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